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Merck
CN

917575

Sigma-Aldrich

PhotoCol-IRG, methacrylated collagen bioink kit, with Irgacure

Synonym(s):

3D Bioprinting, Bioink, Collagen

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.23
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description

Methacrylated collagen:
Degree of methacrylation ≥ 20%

Product components :
Methacrylated collagen (100 mg)
20 mM acetic acid (50 mL)
Neutralization solution (10 mL)
Irgacure photoinitiator ( 100 mg)

Quality Level

sterility

sterile; sterile-filtered

impurities

≤10 EU/mL Endotoxin

storage temp.

2-8°C

Application

PhotoCol-IRG bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included), which allows UV crosslinking of the printed structure at 365 nm. PhotoCol-IRG provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.

Legal Information

PhotoCol is a trademark of Advanced BioMatrix, Inc.

Pictograms

Environment

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids

Regulatory Information

监管及禁止进口产品
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A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues.
Stoecklin C, et al.
Advanced Biosystems (2018)
Abigail Isaacson et al.
Experimental eye research, 173, 188-193 (2018-05-18)
Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However
Kathryn E Drzewiecki et al.
Langmuir : the ACS journal of surfaces and colloids, 30(37), 11204-11211 (2014-09-11)
Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In
Apekshya Chhetri et al.
Current protocols in chemical biology, 11(2), e65-e65 (2019-06-06)
With the increase in knowledge on the importance of the tumor microenvironment, cell culture models of cancers can be adapted to better recapitulate physiologically relevant situations. Three main microenvironmental factors influence tumor phenotype: the biochemical components that stimulate cells, the
Mohammad Izadifar et al.
Tissue engineering. Part C, Methods, 24(2), 74-88 (2017-10-21)
Biofabrication of cell supportive cardiac patches that can be directly implanted on myocardial infarct is a potential solution for myocardial infarction repair. Ideally, cardiac patches should be able to mimic myocardium extracellular matrix for rapid integration with the host tissue

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