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Sigma-Aldrich

TissueFab® bioink kit

(Gel)ma Fibrin (UV/365), low endotoxin

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Synonym(s):
Fibrin, Fibrinogen, GelMA, Gelatin methacrylamide, Gelatin methacrylate, Gelatin methacryloyl, Thrombin

description

HNMR in D2O at 40°C

Quality Level

100

form

(Solid chunks, fibers or powder)

impurities

<10 CFU/g Bioburden (Fungal)
<10 CFU/g Bioburden (Total Aerobic)
<125 EU/g Endotoxin

color

white

storage temp.

2-8°C

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General description

Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. Fibrinogen is a soluble glycoprotein produced by liver. It can be enzymatically polymerized to fibrin monomers by thrombin and then to fibrin based blood clot during blood coagulation. With multi cell binding sites, such as RGD (Arg-Gly-Asp), fibrin plays an important role in cellular-matrix interaction, can promote cell adhesion, proliferation and migration.

Application

The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation. Gelatin methacrylate based bioinks have been used to bioprint osteogenic , chondrogenic , hepatic , adipogenic , vasculogenic , epithelial , endothelial , cardiac valve , skin , tumor and other tissues and constructs. Fibrinogen and fibrin has been widely utilized in tissue engineering for wound healing, neural regeneration, bone generation and vascularization, etc.
TissueFab® bioink kit- (Gel)ma Fibrin (UV/365), low endotoxin is a ready-to-use bioink which is formulated for high cell viability, osteoinduction and printing fidelity and is designed for extrusion-based 3D bioprinting and subsequent crosslinking with exposure to 405nm visible light. TissueFab® bioink kit- (Gel)ma Fibrin (UV/365), low endotoxin can be used with most extrusion-based bioprinters, are biodegradable, and are compatible with human dermal fibroblasts (HDFs). TissueFab® bioink kit- (Gel)ma Fibrin (UV/365), low endotoxin enables the precise fabrication of osteogenic 3D cell models and tissue constructs for research in 3D cell biology, tissue engineering, in vitro tissue models, and regenerative medicine.

The protocol can be found under "More Documents" at the bottom of the page.

TissueFab® bioink kit- (Gel)ma Fibrin (UV/365), low endotoxin contains:
2- 500 mg lyophilized ink components
1- lyophilized thrombin powder
1- 10 ml HEPES buffer.

Features and Benefits

In addition to fast gelation, the methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio. Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies.

Low Endotoxin, low bioburden: Endotoxins have been demonstrated negatively impact cellular growth, morphology, differentiation, inflammation and protein expression. Bioburden is defined as the number of contaminated organisms found in a given amount of material. We test each lot for endotoxins as well as total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/pyrogen-testing/what-is-endotoxin

Legal Information

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Emily Abelseth et al.
ACS biomaterials science & engineering, 5(1), 234-243 (2019-01-14)
3D bioprinting offers the opportunity to automate the process of tissue engineering, which combines biomaterial scaffolds and cells to generate substitutes for diseased or damaged tissues. These bioprinting methods construct tissue replacements by positioning cells encapsulated in bioinks into specific
David B Kolesky et al.
Advanced materials (Deerfield Beach, Fla.), 26(19), 3124-3130 (2014-02-20)
A new bioprinting method is reported for fabricating 3D tissue constructs replete with vasculature, multiple types of cells, and extracellular matrix. These intricate, heterogeneous structures are created by precisely co-printing multiple materials, known as bioinks, in three dimensions. These 3D
Irene Chiesa et al.
Biofabrication, 12(2), 025013-025013 (2020-01-14)
Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included in any reliable and adherent in vitro model platform for the study of bone physiology and pathogenesis
N Laurens et al.
Journal of thrombosis and haemostasis : JTH, 4(5), 932-939 (2006-05-13)
Fibrinogen and fibrin play an important role in blood clotting, fibrinolysis, cellular and matrix interactions, inflammation, wound healing, angiogenesis, and neoplasia. The contribution of fibrin(ogen) to these processes largely depends not only on the characteristics of the fibrin(ogen) itself, but
Harold A Scheraga
Biophysical chemistry, 112(2-3), 117-130 (2004-12-02)
The thrombin-catalyzed conversion of fibrinogen (F) to fibrin consists of three reversible steps, with thrombin (T) being involved in only the first step which is a limited proteolysis to release fibrinopeptides (FpA and FpB) from fibrinogen to produce fibrin monomer.
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