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Merck
CN

940798

TissueFab®PODS Growth Factor Loaded Bioink Kit

Neural differentiation, conductive Vis/405 nm

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description

Application(s): neural differentiation, cell culture, tissue engineering, Bioburden (fungal): < 5 CFU/mL
Bioburden (Total Aerobic): <5 CFU/ml
Endotoxin: <50 EU/ml

form

(viscous liquid or gel)

composition

PODS Human BDNF, 25µg , PODS Human GDNF, 25µg , PODS Human NGF Full-length, 25µg , TissueFab® bioink, conductive, Vis/405 nm, low endotoxin

color

black

pH

6.5-7.5

compatibility

(extrusion-based 3D bioprinting)

storage temp.

−20°C

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General description

Rethink your bioinks with TissueFab® About the material: The TissueFab® PODS Growth Factor Loaded Bioink Kit, Neural differentiation, Vis/405 nm contains: *


  • Low endotoxin conductive bioink
  • Human NGF Full-length PODS
  • Human BDNF PODS
  • Human GDNF PODS
Printing and crosslinking parameters:


  • Suitable for extrusion-based bioprinting
  • Visible light crosslinking at 405 nm wavelength
TissueFab PODS Growth Factor Loaded Bioink kit, neural differentiation, conductive Vis/405nm is a ready-to-use bioink kit which is formulated for high cell viability, printing fidelity, and is designed for extrusion-based 3D bioprinting and subsequent crosslinking with exposure to 405nm visible light. TissueFab PODS Growth Factor Loaded Bioink kit, Liver differentiation, (Gel)MA Vis/405nm can be used with most extrusion-based bioprinters, is biodegradable and cytocompatible. The included bioink component is an electrically conductive bioink for 3D bioprinting applications. It is intended to generate ideal cell environments for neural cells, which enable them to transfer electrical signals, enhancing cell communication and capacity for network formation. It is based on gelatin methacryloyl (GelMA) hydrogel system. Carbon nanotubes (CNTs) are incorporated to introduce conductivity, meanwhile enhance the mechanical properties of GelMA hydrogels.

Included growth facors, NGF, BDNF, and GDNF are known to play crucial roles in regulating neuronal differentiation or promoting neuronal survival. Their inclusion with PODS® delivery system allows their sustained release within bioprinted constructs and retainment of 70% bioactivity after 6 months. When mixed with the bioink component, the included PODS growth factors can promote neural cell differentiation.

More information and a step by step protocol can be found under the "More Documents" section of this page.

Application

TissueFab®PODS Growth Factor Loaded Bioink kit, neural differentiation, conductive Vis/405nm enables the precise fabrication of 3D neural cell models and tissue constructs for research in 3D cell biology, tissue engineering, in vitro tissue models, and regenerative medicine.

Features and Benefits

Key Features and Benefits:

Each TissueFab® bioink is developed and tested to meet strict criteria to best suit researchers′ needs. This includes:

  • Printability and rheological properties
  • Crosslinking
  • Cell viability
  • Biocompatibility
  • Low bioburden
  • Low endotoxin
This product contains 10 ml of bioink solution and growth factors PODS® packaged separately to allow for optimization.

Legal Information

PODS is a registered trademark of Cell Guidance Systems Ltd
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • 926078TissueFab® bioink Conductive, Vis/405 nm, low endotoxinSDS

  • 939242Protein delivery system, Human TGF-β2, PODS®SDS

  • 939137Protein delivery system, Human NGF Full-Length, PODS®SDS

  • 939307Protein delivery system, Human BDNF, PODS®SDS

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

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Brain-derived neurotrophic factor increases cell number of neural progenitor cells derived from human induced pluripotent stem cells
Pansri P, et al.
PeerJ, 9, e11388-e11388 (2021)
Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators
Shin R S, et al.
ACS Nano, 7(3), 2369-2380 (2013)
Differentiation by nerve growth factor (NGF) involves mechanisms of crosstalk between energy homeostasis and mitochondrial remodeling
Martorana F, et al.
Cell Death & Disease, 9(3), 391-391 (2018)
GDNF improves survival and reduces apoptosis in human embryonic dopaminergic neurons in vitro
Clarkson E D, et al.
Cell and Tissue Research, 289(2), 207-210 (1997)

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