F2502
Fluorescein isothiocyanate isomer I
90%
Synonym(s):
FITC, Fluorescein 5-isothiocyanate
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About This Item
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
EC Number:
222-042-0
UNSPSC Code:
12171500
PubChem Substance ID:
Beilstein/REAXYS Number:
1407295
MDL number:
assay
90%
form
powder
mp
>360 °C (lit.)
solubility
acetone: 1 mg/mL
λmax
499 nm
fluorescence
λex 492 nm; λem 518 nm (green)
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
Application
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Other Notes
This product has been replaced by F7250-SIGMA | Fluorescein isothiocyanate isomer I suitable for protein labeling, ≥90% (HPLC), powder
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
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Fluorescent Protein Tracing (1962)
K Weber et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(2), 459-463 (1975-02-01)
Cytoplasmic microtubules in tissue culture cells can be directly visualized by immunofluorescence microscopy. Antibody against tubulin from the outer doublets of sea urchin sperm flagella decorates a network of fine cytoplasmic fibers in a variety of cell lines of human
Fluorescein isothiocyanates: improved synthesis and purity. Spectral studies.
J E Sinsheimer et al.
Analytical biochemistry, 57(1), 227-231 (1974-01-01)