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Merck
CN

L020080

Sigma-Aldrich

TCA Deblock

configured for MerMade, configured for PerkinElmer, configured for Polygen

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About This Item

UNSPSC Code:
12352200
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type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

28-32 g/L (TCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear
clear

compatibility

configured for MerMade
configured for PerkinElmer
configured for Polygen

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Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

WGK

WGK 2

Regulatory Information

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