Cap A

Name: Cap A
Brand: ALDRICH

configured for (ÄKTA^® and OligoPilot^®)

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About This Item

NACRES:

NA.21

UNSPSC Code:

12352200

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Properties

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

impurities

≤100 ppm water content (Karl Fischer)

color

clear

suitability

conforms to structure (GC, Formulation)

compatibility

configured for (ÄKTA^® and OligoPilot^®)

storage temp.

room temp

Description

Application

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Other Notes

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.