Cap B2

Name: Cap B2
Brand: ALDRICH

configured for (ÄKTA^® and OligoPilot^®)

Synonym(s):

Cap B

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About This Item

NACRES:

NA.21

UNSPSC Code:

12352200

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Properties

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

storage condition

dry at room temperature

impurities

≤200 ppm water content (Karl Fischer)

color

clear

suitability

conforms to structure (GC, Formulation)

compatibility

configured for (ÄKTA^® and OligoPilot^®)

Description

General description

Cap B solution, containing tetrahydrofuran (THF), pyridine and N-methylimidazole (NMI), is mixed in situ with Cap A solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.Tetrahydrofurane has been replaced by acetonitrile in ÄKTA Cap B1 and B2.Cap B is configured for ÄKTA formulation.

Preparation Note

Acetonitrile / Pyridine 4/6 (v/v)

Other Notes

Cap B2 solution, containing acetonitrile (ACN) and pyridine, is mixed in situ with Cap A and Cap B1 solution during the capping (acetylation) reaction.
Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.