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QBD10193

Sigma-Aldrich

Biotin-dPEG®3-NH3+TFA-

>95% (HPLC)

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Synonym(s):
Biotin-PEG-Amine, Biotin-PEG3-NH3-TFA-, Biotin-PEG3-amine TFA salt, Biotin-PEG3-ammonium trifluoroacetate
Empirical Formula (Hill Notation):
C22H39F3N4O7S
Molecular Weight:
560.63

Assay

>95% (HPLC)

form

solid or viscous liquid

reaction suitability

reaction type: Biotinylations
reaction type: Pegylations

polymer architecture

shape: linear
functionality: monofunctional

shipped in

ambient

storage temp.

−20°C

Features and Benefits

Biotin-dPEG3-NH3+TFA- (Biotin-dPEG3-ammonium trifluoroacetate) reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under mildly basic conditions. It can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Also, the product can react with carbonyl groups (aldehydes or ketones) to form a Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary. Biotin-dPEG3-NH3+TFA- can be used to biotinylate antibodies useful in western blotting and immunohistochemical applications.

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Legal Information

Products Protected under U.S. Patent #s 7,888,536 & 8,637,711 and European Patent #s 1,594,440 & 2,750,681
dPEG is a registered trademark of Quanta BioDesign

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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