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ZYMD4202

ZymoPURE II Plasmid Maxiprep Kit

High yield, Endotoxin-free, sufficient for 10 purifications

Synonym(s):

DNA Isolation, Fast maxiprep, High-Yield plasmid, Maxiprep, Maxiprep endofree, Plasmid purification

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About This Item

UNSPSC Code:
41105512
Technique(s):
DNA purification: suitable
Storage temp.:
room temp
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manufacturer/tradename

D4202

packaging

pkg of 10 purifications

technique(s)

DNA purification: suitable

test parameters

total test time: ≤ 18 min

storage temp.

room temp

General description

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Maxiprep kit can process up to 150 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

Application

DNA plasmid purification

Features and Benefits

  • Fast : Perform plasmid maxipreps in only 16 minutes using a simple spin-column protocol.
  • High Yield: Up to 3 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 µg.
  • Ultra-Pure: Eluted plasmid DNA is Endotoxin-Free and Transfection Ready.

Legal Information

ZymoPURE is a trademark of Zymo Research


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Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 3

Regulatory Information

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