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About This Item
UNSPSC Code:
41105512
Technique(s):
DNA purification: suitable
Storage temp.:
room temp
manufacturer/tradename
D4202
packaging
pkg of 10 purifications
technique(s)
DNA purification: suitable
test parameters
total test time: ≤ 18 min
storage temp.
room temp
General description
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE™ II Maxiprep kit can process up to 150 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE™ II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Application
DNA plasmid purification
Features and Benefits
- Fast : Perform plasmid maxipreps in only 16 minutes using a simple spin-column protocol.
- High Yield: Up to 3 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 µg.
- Ultra-Pure: Eluted plasmid DNA is Endotoxin-Free and Transfection Ready.
Legal Information
ZymoPURE is a trademark of Zymo Research
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 3
Regulatory Information
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