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About This Item
UNSPSC Code:
41105518
Technique(s):
RNA purificationSpin-column
Elution Volume: > 25 µl
Size Range: Total RNA > 17 nt: suitable using Any sample stored and preserved in TRI Reagent®,TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA).
Elution Volume: > 25 µl
Size Range: Total RNA > 17 nt: suitable using Any sample stored and preserved in TRI Reagent®,TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA).
Storage temp.:
room temp
manufacturer/tradename
R2050
packaging
pkg of 50 purifications
technique(s)
RNA purificationSpin-column
Elution Volume: > 25 µl
Size Range: Total RNA > 17 nt: suitable using Any sample stored and preserved in TRI Reagent®,TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA).
storage temp.
room temp
General description
The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.
Application
Total RNA purification
Features and Benefits
- Fast : RNA from Trizol ready in 7 minutes. Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Unbiased: Complete RNA recovery without miRNA loss.
Legal Information
Direct-zol is a trademark of Zymo Research
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
TRIzol is a registered trademark of Molecular Research Center, Inc.
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signalword
Danger
hcodes
Hazard Classifications
Flam. Liq. 3 - Resp. Sens. 1
Storage Class
3 - Flammable liquids
wgk
WGK 3
Regulatory Information
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