Merck
CN
All Photos(1)

Documents

Safety Information

100500C

Avanti

E. coli Extract Total

Avanti Polar Lipids

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
E. coli Total Lipid Extract
CAS Number:
1240502-50-4

form

chloroform solution

packaging

pkg of 2 × 20 mL (100500C-1g)
pkg of 1 × 4 mL (100500C-100mg)
pkg of 5 × 4 mL (100500C-500mg)

manufacturer/tradename

Avanti Polar Lipids

concentration

25 mg/mL (100500C-100mg)
25 mg/mL (100500C-1g)
25 mg/mL (100500C-500mg)

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total is suitable:
  • for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
  • to prepare E. coli liposomes for liposomal content release assay
  • for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

E. coli extract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.
E. coli Extract Total has been used:
  • in the preparation of small unilamellar vesicles (SUVs)
  • as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
  • in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

Biochem/physiol Actions

The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)
5 mL Clear Glass Sealed Ampule (100500C-100mg)
5 mL Clear Glass Sealed Ampule (100500C-500mg)

also commonly purchased with this product

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

危险化学品
易制毒化学品（2类）

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores
Kim JS, et al.
Biochemical and biophysical research communications, 467-472 (2018)
In vitro systems for Atg8 lipidation
Zens B, et al.
Methods, 75, 37-43 (2015)
Effects of lipid environment on the conformational changes of an ABC importer
Rice AJ, et al.
Biochemical and biophysical research communications, 8(4), 327-333 (2014)
Variation of the Detergent-Binding Capacity and Phospholipid Content of Membrane Proteins When Purified in Different Detergents
Ilgu H, et al.
Biophysical Journal, 106, 1660-1670 (2014)
Regulation of the Function of the Human ABCG2 Multidrug Transporter by Cholesterol and Bile Acids: Effects of Mutations in Potential Substrate and Steroid Binding Sites
Telbisz A, et al.
Drug Metabolism and Disposition, 42(4), 575?585-575?585 (2014)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service