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Merck
CN

100500C

Avanti

E. coli Extract Total

Avanti Research™ - A Croda Brand

Synonym(s):

E. coli Total Lipid Extract

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About This Item

CAS Number:
1240502-50-4
MDL number:
MFCD18427707
NACRES:
NA.25
UNSPSC Code:
12352211

Key Documents

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Product Name

E. coli Extract Total, Avanti Research™ - A Croda Brand

assay

>99% (TLC)

form

chloroform solution

packaging

pkg of 2 × 20 mL (100500C-1g)
pkg of 1 × 4 mL (100500C-100mg)
pkg of 5 × 4 mL (100500C-500mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

concentration

25 mg/mL (100500C-100mg)
25 mg/mL (100500C-1g)
25 mg/mL (100500C-500mg)

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)
5 mL Clear Glass Sealed Ampule (100500C-100mg)
5 mL Clear Glass Sealed Ampule (100500C-500mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Application

E. coli Extract Total is suitable:
  • for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
  • to prepare E. coli liposomes for liposomal content release assay
  • for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

E. coli extract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.
E. coli Extract Total has been used:
  • in the preparation of small unilamellar vesicles (SUVs)
  • as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
  • in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Biochem/physiol Actions

The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

wgk

WGK 3

Regulatory Information

易制毒化学品（2类）
危险化学品
动植物源性产品
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In vitro systems for Atg8 lipidation
Zens B, et al.
Methods, 75, 37-43 (2015)
GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores
Kim JS, et al.
Biochemical and biophysical research communications, 467-472 (2018)
Variation of the detergent-binding capacity and phospholipid content of membrane proteins when purified in different detergents
Ilgu H, et al.
Biophysical Journal, 106(8), 1660-1670 (2014)
Regulation of the Function of the Human ABCG2 Multidrug Transporter by Cholesterol and Bile Acids: Effects of Mutations in Potential Substrate and Steroid Binding Sites
Telbisz A, et al.
Drug Metabolism and Disposition, 42(4), 575?585-575?585 (2014)
Variation of the Detergent-Binding Capacity and Phospholipid Content of Membrane Proteins When Purified in Different Detergents
Ilgu H, et al.
Biophysical Journal, 106, 1660-1670 (2014)
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