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330021S

Avanti

WR304 mAb

Avanti Research™ - A Croda Brand

Synonym(s):

WR304 mAb, WR304 Mouse Monoclonal Antibody (IgM), Anti-(PIP/PIP2), 1X TBS (Tris Buffered Saline) with 1mM Na2 EDTA

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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Product Name

WR304 mAb, Avanti Research™ - A Croda Brand 330021S

antibody form

purified immunoglobulin

antibody product type

primary antibodies

Assay

>95%

packaging

pkg of 1 × 0.1 mL (polypropylene tube with screw cap (330021S-100ug))

manufacturer/tradename

Avanti Research™ - A Croda Brand 330021S

concentration

1 mg/mL (330021S-100ug)

shipped in

dry ice

storage temp.

−70°C

General description

WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways. This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti′s Lipid A.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.

Immunogen

Antigens: Brain PI(4)P

Application

ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.

The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).

Biochem/physiol Actions

WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.

Packaging

0.5 mL Polypropylene Tube with Screw Cap (330021S-100ug)

Preparation Note

Store undiluted at either -20°C or -80°C.

Other Notes

Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
Ig Class: Mouse IgM (kappa).
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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N M Wassef et al.
Molecular immunology, 21(10), 863-868 (1984-10-01)
Monoclonal antibodies against phosphatidylinositol phosphate were produced after injecting a mouse with liposomes containing dimyristoylphosphatidylcholine, cholesterol, phosphatidylinositol phosphate and lipid A. The antibodies raised were IgM (kappa) and their activities were assayed by complement-dependent damage to liposomes lacking lipid A
Bruce K Brown et al.
Journal of virology, 81(4), 2087-2091 (2006-12-08)
Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked
Gary R Matyas et al.
Biochimica et biophysica acta, 1788(3), 660-665 (2008-12-23)
Two human mAbs (2F5 and 4E10), originally derived from HIV-1-infected patients, are important, but rare, mAbs that exhibit broad cross-clade neutralizing activities against HIV-1. In addition to peptide sequences on the gp41 envelope protein, both antibodies reportedly also bound specifically
Zoltan Beck et al.
Biochemical and biophysical research communications, 354(3), 747-751 (2007-01-30)
The binding characteristics of two monoclonal antibodies (mAb) to phosphatidylinositol-4-phosphate (PIP) were examined: a murine IgM mAb to PIP; and a human IgG mAb (4E10) that binds both to HIV-1 envelope protein and also to neutral and anionic phospholipids, including

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