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03-0055-00

Millipore

SMC® Human Interleukin 8 (IL-8) Immunoassay Kit

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eCl@ss:
32161000

species reactivity

human

Quality Level

200

manufacturer/tradename

SMC®

assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

Related Categories

General description

Interleukin-8 (IL-8) is a CXC chemokine synthesized by many cell types, including macrophages, epithelial cells and endothelial cells in response to a pathogen or inflammatory stimulus. IL-8 functionendothelials as a chemotactic factor that attracts neutrophils, basophils, and T-cells. IL-8 is one of the major mediators of the inflammatory response and of innate immunity. Its function is to recruit neutrophils to phagocytose the foreign antigen. IL-8 is also a potent angiogenic factor that induces neutrophils to leave the bloodstream and enter into the surrounding tissue. IL-8 has been shown to have roles in the pathological angiogenesis that is associated with solid tumors, diabetic retinopathy and rheumatoid arthritis (RA).
The SMC® Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® IL-8 Immunoassay Kit unparalleled performance in the quantification of IL-8 in human EDTA plasma and serum.
Used to detect/quantify:IL-8

Components

Human IL-8 Coated Beads
Standard Diluent
Human IL-8 Detection Antibody
Assay Buffer
Human IL-8 Standard
SMC® Human IL-8 Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D

Analysis Note

Low-end CV% Range: 6 - 18%|Low-end CV% Average: 12%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Information

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