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Merck
CN

04-1552

Anti-TLS Antibody, clone 1FU-1D2

ascites fluid, clone 1FU-1D2, from mouse

Synonym(s):

75 kDa DNA-pairing protein, FUS-CHOP fusion protein, FUS-CHOP protein fusion, Fusion gene in myxoid liposarcoma, Oncogene FUS, Oncogene TLS, Translocated in liposarcoma protein, fus-like protein, fus/tls-chop oncogene, fusion (involved in t(12, 16) in ma

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1FU-1D2, monoclonal

species reactivity

human, rat, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_004951.1

UniProt accession no.

P35637

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... FUS(2521)

General description

Observed at 65 kDa
The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.

Immunogen

Epitope: Unknown
Linear peptide corresponding to human TLS.

Application

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected TLS in C2C12 cells.
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Transcription Factors
Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.

Biochem/physiol Actions

This antibody recognizes TLS.

Physical form

Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HepG2 cell lysate
Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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