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Merck
CN

05-1560-M

Anti-PI3 Kinase-C2-alpha Antibody, clone 9H4.2

clone 9H4.2, from mouse

Synonym(s):

phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, PI3K-C2alpha, Phosphoinositide 3-kinase-C2-alpha, PtdIns-3-kinase C2 subunit alpha, phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit a

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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biological source

mouse

antibody product type

primary antibodies

clone

9H4.2, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_002636.2

UniProt accession no.

O00443

Quality Level

100

Gene Information

human ... PIK3C2A(5286)

General description

PI3 Kinase, class II a (PI3KC2A) is also known as phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha. It plays an important role in vesicular transport in eukaryotic cells. PI3 Kinase-C2-alpha also plays a part in cellular proliferation and survival.
~190 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human PI3K-C2-alpha.

Application

Anti-PI3 Kinase-C2-alpha Antibody, clone 9H4.2 detects level of PI3 Kinase-C2-alpha & has been published & validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PI3K-C2-alpha in A431 cells.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Physical form

Format: Purified
Ion Exchange
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate
Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3K-C2-alpha on 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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