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Merck
CN

05-1573

Sigma-Aldrich

Anti-Pax5 Antibody, clone 1H9

clone 1H9, from rat

Synonym(s):

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H9, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_032808

UniProt accession no.

Q02650

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PAX5(5079)

General description

Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.
~42 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to mouse Pax5.

Application

Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE517

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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