05-443
Anti-VEGF Antibody, clone JH
clone JH, Upstate®, from mouse
Synonym(s):
Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-VEGF Antibody, clone JH, clone JH, Upstate®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JH, monoclonal
species reactivity
rat, mouse, rabbit, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable (paraffin)
isotype
IgG1κ
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VEGFA(7422)
Preparation Note
Stable for 1 year at from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.
Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Application
Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.
Biochem/physiol Actions
Other species not tested.
Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
34-54 kDa isoforms
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Immunogen
Recombinant human VEGF121. Clone JH121
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hiroshi Kubo et al.
Genes to cells : devoted to molecular & cellular mechanisms, 14(3), 407-424 (2009-02-21)
Although ex vivo expanded mesenchymal stem cells (MSC) have been used in numerous studies, the molecular signature and in vivo distribution status of MSC remain unknown. To address this matter, we identified numerous human MSC-characteristic genes--including nine transcription factor genes--using
Treatment-induced neoangiogenesis in cerebral arteriovenous malformations.
U Sure, N Butz, A M Siegel, H D Mennel, S Bien, H Bertalanffy
Clinical Neurology and Neurosurgery null
VEGF121, a vascular endothelial growth factor (VEGF) isoform lacking heparin binding ability, requires cell-surface heparan sulfates for efficient binding to the VEGF receptors of human melanoma cells.
Cohen, T, et al.
The Journal of Biological Chemistry, 270, 11322-11326 (1995)
C O Tibesku et al.
The open orthopaedics journal, 5, 177-180 (2011-06-03)
To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA). In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12
M Wartenberg et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(6), 995-1005 (2001-04-09)
Tumor vascularization is the rate-limiting step for the progression of cancer. Differential steps of tumor-induced angiogenesis were studied by a novel in vitro confrontation culture of avascular multicellular prostate tumor spheroids and embryoid bodies grown from pluripotent embryonic stem (ES)
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