Serine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.

The unconjugated antibody (Cat. No. 05-430) is shown to recognize the ~370 kDa phosphoATM target band and a >400 kDa non-specific band.

Epitope: pSer1981.

KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence is 11/12 identical to the corresponding mouse and rat sequence.

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor^™ 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

The unconjugated antibody (Cat. Nos. 05-740, MAB3806 & MAB3806-C) is shown to be suitable also for ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting applications. PE-conjugate (Cat. No. FCMAB110P) is also available and tested for Flow cytometry application.

Reacts with ATM phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.

Protein G Purified

Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected UV-induced ATM Ser1981 phosphorylation in HeLa cells.

Concentration: Please refer to lot specific datasheet.

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