95 kDa

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.

Epitope: Tyr694/Tyr699

KLH-conjugated linear peptide corresponding to phosphorylated human STAT5A/B at and around Tyr694/Tyr699.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Transcription Factors

Use Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2 (rabbit monoclonal antibody) validated in WB to detect phospho-STAT5A/B (Tyr694/699) also known as signal transducer & activator of transcription 5A/B.

Recognizes phosphorylated STAT5A/B atTyr694/Tyr699.

Format: Purified

Protein A purified

Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 30% Glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
GMCSF treated and untreated U937 cell lysate

Evaluated by Western Blot in U937 cell lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected STAT5 A/B on 10 µg of U937 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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