Centrosomal protein 63 (Cep63) serves an ATM and ATR substrate for spindle assembly activation following chromosomal breakage. It is shown that inactivation of the CEP63 gene in avian DT40 cells impairs spindle assembly and prevents ATM- and ATR-dependent effects on mitosis. Cep63 is also reported to bind to and recruit Cdk1 to centrosomes and is thought to be involved the regulation mitotoic entry, centrosome amplification, and genome maintentance.

~63 kDa observed. Different isoforms or uncharacterized bands may be observed in some endogenous cell lysates. Experiments using transfected cells and siRNA have confirmed detection of Cep63.

Recombinant protein corresponding to human Cep63.

Immunocytochemistry Analysis: A representative lot detected Cep63 in U20S cells. (Nicola Brown, CRUK Clare Hall Laboratories, London, UK)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-Cep63 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Cep63 also known as Centrosomal protein of 63 kDa.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK 293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1: 500 dilution of this antibody detected Cep63 in 10 µg of HEK 293 cell lysate.

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