68 kDa

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.

This antibody recognizes Paxillin phosphorylated at Ser126.

Epitope: Ser126

KLH-conjugagted synthetic peptide corresponding to human Paxillin at phosphorylated Ser126.

Anti-phospho-Paxillin (Ser126) Antibody is an antibody against phospho-Paxillin (Ser126) for use in WB.

Research Category
Signaling

Research Sub Category
G-proteins

Replaces: AB3793

Affinity purified

PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.

Maintain at -20°C for up to one year or 2-8°C for short term use from date of receipt. Avoid repeated freeze/thaw cycles.

Control
Pervandate treated and untreated Huvec cell lysate

Evaluated by Western Blot in pervandate treated and untreated Huvec cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Paxillin on 10 µg of Pervandate treated and untreated Huvec cell lysate.

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