Skip to Content
Merck
CN

Key Documents

View All Documentation

1.04192

Trypan Blue

Synonym(s):

Direct blue 14

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
C34H24N6O14S4Na4
CAS Number:
72-57-1
Molecular Weight:
960.81
NACRES:
NA.21
Beilstein/REAXYS Number:
4360496
MDL number:
MFCD00003969
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solid

Quality Segment

200

potency

6200 mg/kg LD50, oral (Rat)

composition

Dye, ≥80% spectrophotometric assay

pH

9.8 (20 °C, 10 g/L in H2O)

mp

>300 °C (lit.)

solubility

10 g/L

bulk density

800 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

General description

Trypan blue (C.I. 23850) for microscopy, is a dry dye for the preparation of a color staining solution, staining histological sample material of human origin, e.g. trichrome staining in collagenous connective tissue. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 590 - 600 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 650 - 820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Still not finding the right product?

Explore all of our products under Trypan Blue

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Regulatory Information

新产品

This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library