InChI key
HJAYEODIXUYVIC-LTFAAIOASA-N
InChI
1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
composition
Dye, ≥80% spectrophotometric assay
mp
182-186 °C
bulk density
270 kg/m3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
General description
The dry dye Oil red O (C.I. 26125) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues, in joint pathology or in cytological sample material. This Oil red O solution is ready-to-use and results in a brilliant, bright red staining of lipids, while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Cat. no. 105175).
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids.
As Certistain® dye it is chemically quality-certified according to highest standards and strict specifications.
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids.
As Certistain® dye it is chemically quality-certified according to highest standards and strict specifications.
Application
For microscopy Certistain®
Analysis Note
<tr><td width=\25%\>Dye content (spectrophotometrically)</td><td width=\75%\>≥ 80 %</td></tr><tr><td width=\25%\>Identity (UV/VIS-Spectrum)</td><td width=\75%\>passes test</td></tr><tr><td width=\25%\>Absorption maximum λ max (chloroform)</td><td width=\75%\>528 - 534 nm</td></tr><tr><td width=\25%\>Spec. Absorptivity A 1%/1cm (λ<SUB>m</SUB><SUB>a</SUB><SUB>x</SUB>; 0.01 g/l; chloroform)</td><td width=\75%\>725 - 910</td></tr><tr><td width=\25%\>TLC-Test</td><td width=\75%\>passes test</td></tr><tr><td width=\25%\>Loss on drying (110 °C)</td><td width=\75%\>≤ 8 %</td></tr><tr><td width=\25%\>Suitability for microscopy</td><td width=\75%\>passes test</td></tr>
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
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