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Merck
CN

1.05231

Fuchsin acid (C.I. 42685)

for microscopy Certistain®

Synonym(s):

Fuchsin acid (C.I. 42685)

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About This Item

Empirical Formula (Hill Notation):
C20H17N3Na2O9S3
CAS Number:
Molecular Weight:
585.54
UNSPSC Code:
41116130
EC Index Number:
221-816-5
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Quality Level

form

solid

composition

Dye, ≥60% spectrophotometric assay

pH

3-4 (20 °C, 10 g/L in H2O)

mp

>130 °C (decomposition)

solubility

10 g/L

bulk density

920 kg/m3

εmax

780-1310 at 540-549 nm in hydrochloric acid at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,23H,21-22H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2

InChI key

WZRZTHMJPHPAMU-UHFFFAOYSA-L

General description

Fuchsin acid (C.I. 42685) for microscopy Certistain®, is a triarylmethane dye used, among others, in trichrome connective tissue stainings according to Mallory and van Gieson. Mallory developed a staining procedure for visualization of collagenous connective tissue, which has been modified and improved over the years.It enables a good differentiation of individual tissue components, provided that the tissue has been fixed in Zenker′s solution. As Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Hydrochloric acid 0.0025 mol/l): 540 - 549 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; hydrochloric acid 0.0025 mol/l): 780 - 1310
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

This item has



Certificates of Analysis (COA)

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