113000
Acridine Orange - CAS 65-61-2 - Calbiochem
Synonym(s):
Acridine Orange - CAS 65-61-2 - Calbiochem, AO, 3,6- bis(Dimethylamino)acridine, HCl
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About This Item
CAS Number:
UNSPSC Code:
12352200
Assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
burnt orange
solubility
DMF: 1 mg/mL
H2O: 1 mg/mL
ethanol: 1 mg/mL
shipped in
ambient
storage temp.
15-25°C
InChI
1S/C17H19N3.ClH/c1-19(2)13-8-9-15-12(10-13)11-14-16(18-15)6-5-7-17(14)20(3)4;/h5-11H,1-4H3;1H
InChI key
ASKSWIKIIFUEOI-UHFFFAOYSA-N
General description
A cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
A cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Interacts with DNA and RNA
Interacts with DNA and RNA
Product does not compete with ATP.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
Other Notes
Gonzalez, K., et al. 1995. Curr. Eye Res.14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
监管及禁止进口产品
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Simultaneous analysis of cellular RNA and DNA content.
Z Darzynkiewicz
Methods in cell biology, 41, 401-420 (1994-01-01)
D K Hermansen et al.
Cytometry, 10(6), 739-742 (1989-11-01)
The sensitivity of bladder wash flow cytometry (BWFCM), voided urinary cytology (VUC), and cytology of catheterized urine obtained at the time of cystoscopy (CUC) were reviewed on all women evaluated for bladder cancer at Memorial Sloan-Kettering Cancer Center between June
G L Busch et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(19), 9165-9169 (1994-09-13)
Cell swelling is shown to induce an increase in acridine orange fluorescence intensity, an effect pointing to the alkalinization of acidic vesicles. Since autophagic hepatic proteolysis is accomplished by pH-sensitive proteinases within acidic lysosomes, this effect may contribute to the
F Lopez et al.
Cytometry, 12(1), 42-49 (1991-01-01)
The antibody Ki67 is currently used to evaluate the proliferative fraction of solid tumors and some hematological malignancies. We have used phytohemagglutinin (PHA)-stimulated peripheral blood lymphocytes as a model to study the entry of quiescent cells into cell cycle and
C A Muro-Cacho et al.
Journal of immunology (Baltimore, Md. : 1950), 154(10), 5555-5566 (1995-05-15)
The occurrence of in vivo apoptosis was investigated in lymph node sections obtained from HIV-infected persons at different stages of disease. The degree of apoptosis in lymph nodes from HIV-infected individuals was compared with that observed in lymph nodes obtained
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