119101
Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, canine, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
General description
This product has been discontinued.
Recognizes the α-subunit of H+,K+-ATPase.
Anti-H+,K+-ATPase, α-Subunit (1019-1034), rabbit polyclonal, recognizes the α-subunit of H+,K+-ATPase. It is validated for use in ELISA, WB, ICC, and IHC (paraffin sections).
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-subunit of H+,K+-ATPase.
Immunogen
Porcine
a synthetic peptide (GVRCCPGSWWDQGLYY) corresponding to amino acids 1019-1034 of porcine H+, K+-ATPase α-subunit
Application
ELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Paraffin Sections (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Paraffin Sections (see comments)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.
Other Notes
Exhibits some cross-reactivity with Na+, K+-ATPase at working dilution for H+,K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Smolka, A. 1992. Biophys. Biochem. Acta 1108, 75.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10-13 - German Storage Class 10 to 13
Regulatory Information
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Pawel J Schweiger et al.
Scientific reports, 8(1), 15255-15255 (2018-10-17)
The processes involved in renewal of the epithelium that lines the mouse stomach remain unclear. Apart from the cells in the isthmus, several other populations located deeper in the gastric glands have been suggested to contribute to the maintenance of
Ivana Novak et al.
The Journal of biological chemistry, 286(1), 280-289 (2010-10-28)
Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma
Ping He et al.
Molecular medicine reports, 11(3), 1911-1916 (2014-11-19)
Curcumin is known to possess anti‑inflammatory properties. Despite the fact that curcumin is known to be a strong inhibitor of H+, K+‑ATPase activity, the mechanism underlying the curcumin‑induced inhibition of the transcription of the H+, K+‑ATPase α subunit in gastric