12-309
HeLa Nuclear Extract
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About This Item
UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.41
biological source
human
Quality Level
form
liquid
manufacturer/tradename
Upstate®
concentration
2.0 mg/mL
technique(s)
western blot: suitable
shipped in
dry ice
General description
Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.
Biochem/physiol Actions
HeLa
Physical form
RIPA sample buffer containing inhibitors. Frozen solution.
Preparation Note
Stable for 6 months at -70°C from date of shipment.
Analysis Note
Routinely evaluated as a positive control for many of Upstates antibodies.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
高风险级别生物产品-Merck
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Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major
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