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14-352-D

Sigma-Aldrich

cRAF (RAF1)

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eCl@ss:
32160405

Assay

≥70%

specific activity

158 pmol/min-μg

concentration

0.71 mg/mL

technique(s)

activity assay: suitable

UniProt accession no.

P04049

Gene Information

human ... RAF1(5894)

General description

FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.

Quality

Evaluated by Kinase assay: 10 μM ATP, 1 μM MEK1(K97R), 1 hour.

Physical properties

65 kDa

Physical form

40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT

Storage and Stability

Stable for > 6 month at –80°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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