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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4G10, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to fluorescein isothiocyanate (FITC). Patent pending.
Recognizes: tyrosine-phosphorylated proteins from all species
Molecular Weight: dependent upon the molecular weight of the tyrosine phosphorylated protein being detected
Species Reactivity: wide range
Recognizes: tyrosine-phosphorylated proteins from all species
Molecular Weight: dependent upon the molecular weight of the tyrosine phosphorylated protein being detected
Species Reactivity: wide range
Immunogen
Phosphotyramine-KLH
Application
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This Anti-Phosphotyrosine Antibody, recombinant clone 4G10, FITC conjugate is validated for use in IC for the detection of Phosphotyrosine.
Physical form
100µg FITC-conjugated recombinant mouse IgG2bκ in 67μl of PBS containing 0.05% sodium azide. Liquid at 4°C.
Preparation Note
9 months at 4°C
Analysis Note
routinely evaluated in immunocytochemistry Immunocytochemistry to detect phosphotyrosine-containing proteins in EGF-stimulated human A431 cells
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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