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Merck
CN

17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.41
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Quality Level

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Related Categories

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Analysis Note

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Other Notes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Demethylase activity is directed by histone acetylation
Cervoni, N. and Szyf, M.
The Journal of Biological Chemistry, 276, 40778-40787 (2001)
Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites
Zhang, J., et al
The Journal of Biological Chemistry, 278, 29192-29200 (2003)
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
M Vignali et al.
The EMBO journal, 19(11), 2629-2640 (2000-06-03)
We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro competition assay was developed to acetylate and transcribe a nucleosomal array template in the presence
Epigenetic regulation of the endothelial nitric oxide synthase gene in persistent pulmonary hypertension of the newborn rat.
Xu, XF; Ma, XL; Shen, Z; Wu, XL; Cheng, F; Du, LZ
Journal of Hypertension null

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