17-441
Rac1/Cdc42 Activation Assay Kit
The Rac1/Cdc42 Activation Assay provides an effective method for detecting Rac & Cdc42 activity in cell lysates.
Synonym(s):
Rac1 activation assay
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About This Item
Quality Level
species reactivity
human, mouse, rat
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
affinity binding assay: suitable (G-protein)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... RAC1(5879)
General description
Biochem/physiol Actions
Anti-Rac1, clone 23A8: Species Cross-reactivity: Human, mouse and rat. Other species cross-reactivity unknown.
Anti-cdc42 Species Cross-reactivity: Human, mouse, rat and dog. Other species cross-reactivity unknown.
Preparation Note
Analysis Note
Other Notes
Anti-Rac1, clone 23A8, Catalog #05-389: One vial containing 250 μg of protein G purified mouse IgG2b in 250 μL of storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide).
Anti-cdc42, (mouse monoclonal IgG1) , Catalog # 05-542: One vial containing 50 μg of purified mouse IgG1 in 200 μL of 50% storage buffer (20 mM sodium phosphate, pH 7.5, 150 mM NaCl, 1.5 mM sodium azide containing, 1 mg/mL BSA) and 50% glycerol.
Mg2+ Lysis/Wash Buffer, 5X, Catalog # 20-168 : Two vials, each vial containing 18 mL of 5X MLB: 125 mM HEPES, pH 7.5, 750 mM NaCl, 5% Igepal CA-630, 50 mM MgCl2, 5 mM EDTA and 10% glycerol.
100X GTPγS, 10mM, Catalog # 20-176: One vial containing 50 μL of 10 mM GTPγS, 100X stock, in 50 mM Tris-HCl, pH 7.8, non-hydrolyzable analog of GTP. Sufficient to label 5 mL of cell lysates.
100X GDP, 100mM, Catalog # 20-177: One vial containing 50 μL of 100 mM GDP, 100X stock, in 50 mM Tris-HCl, pH 7.8. GDP (Guanosine 5′-Diphosphate) for in vitro labeling of G-proteins in the inactive form. Sufficient to label 5ml of cell lysates.
Legal Information
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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