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203118

Biotin-NHS, Water-Soluble

Name: Biotin-NHS, Water-Soluble
Brand: MM

Synonym(s):

Biotin-NHS, Water-Soluble, Sulfobiotin-NHS, Sulfosuccinimidobiotin

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₈N₃O₈S₂ · Na

Molecular Weight:

443.43

NACRES:

NA.41

UNSPSC Code:

41116133

Form:

solid

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Properties

biological source

(Synthetic chemical)

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated

color

off-white

solubility

DMSO: 10 mg/mL, distilled water: 4 mg/mL

shipped in

ambient

storage temp.

2-8°C

Description

General description

Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Preparation Note

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Analysis Note

homogeneous single spot by TLC

Other Notes

Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c