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Key Documents

203351-M

Millipore

Blasticidin S, Hydrochloride, Streptomyces sp., Sterile-Filtered Aqueous Solution, Cell Culture-Tested

sterile; sterile-filtered, suitable for cell culture

Synonym(s):

Blasticidin S, Hydrochloride, Streptomyces sp., Sterile-Filtered Aqueous Solution, Cell Culture-Tested

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About This Item

Empirical Formula (Hill Notation):
C17H26N8O5 · xHCl
CAS Number:
3513-03-9
Molecular Weight:
422.44 (free base basis)
MDL number:
MFCD02091640
UNSPSC Code:
12352200

sterility

sterile; sterile-filtered

Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

General description

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative.

Warning

Toxicity: Highly Toxic (H)

Physical form

Supplied at 10 mg/ml in 20 mM HEPES, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Kimura, M., et al. 1994. Biochim. Biophys. Acta1219, 653.
Kimura, M., et al. 1994. Mol. Gen. Genet.242, 121.
Kojima, N., et al. 1994. J. Biol. Chem.269, 30451.
Kudo, T., et al. 1994. J. Immunol. Methods177, 17.
Sutoh, K. 1993. Plasmid30, 150.
Izumi, M., et al. 1991. Exp. Cell Res.197, 229.
Yamaguchi, H., et al. 1965. J. Biol. Chem.57, 667.
Takeuchi, S., et al. 1958. J. Antibiot.11, 1.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301

Hazard Classifications

Acute Tox. 3 Oral

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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