208910
Anti-Calreticulin (405-417) Rabbit pAb
liquid, Calbiochem®
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
monkey, human, mouse, canine, hamster, bovine, rat, porcine
Application:
—
Citations:
4
biological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
monkey, human, mouse, canine, hamster, bovine, rat, porcine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry )
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.
Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Immunogen
Human
a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
Application
Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Physical form
In PBS, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 208910-50UL | 04055977219524 |