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Sigma-Aldrich

Anti-CD44v10 Rabbit pAb

liquid, Calbiochem®

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biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

canine, porcine, rabbit, mouse, human, bovine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)

General description

Anti-CD44v10, rabbit polyclonal, recognizes CD44v10 in endothelial cells. It is validated for ELISA, FC, IP, RIA, WB, and IHC on frozen and paraffin sections.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) chromatography. Recognizes the CD44v10 protein.
Recognizes CD44v10 in endothelial cells.

Immunogen

Human
a synthetic peptide corresponding to amino acids from human CD44v10

Application

ELISA (1:2000)

Flow Cytometry (1:1000)

Frozen Sections (1:250)

Immunoblotting (1:10,000)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Paraffin Sections (1:250)

Radioimmunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Lokeshwar, V.B., et al. 1996. J. Biol. Chem.271, 23853.
Dall, P., et al. 1994. Cancer Res.54, 3337.
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA89, 12160.
Arch, R., et al. 1992. Science275, 682.
Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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25G
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Steven J Wang et al.
The Laryngoscope, 119(8), 1518-1530 (2009-06-10)
The CD44 family of receptors includes multiple variant isoforms, several of which have been linked to malignant properties including migration, invasion, and metastasis. The objective of this study was to investigate the role of the CD44 v3, v6, and v10

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