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About This Item
Empirical Formula (Hill Notation):
C72H78N10O27
Molecular Weight:
1515.44
UNSPSC Code:
12352200
Quality Level
Assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
protect from light
color
yellow
solubility
DMSO: 1 mg/mL
fluorescence
λex ~485 nm
λem ~535 nm
shipped in
wet ice
storage temp.
−20°C
General description
A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate
A highly sensitive, photostable caspase-3 fluorogenic substrate
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110
Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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