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CELLULYSIN® Cellulase, Trichoderma viride

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Synonym(s):
β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
MDL number:
MFCD00081510

form

solid

specific activity

≥10,000 units/g dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow-white

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Application

CELLULYSIN® Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
  • in the enzyme mix used for in situ hybridization to plant chromosomes
  • in the enzyme solution for the transformation of Phytophthora species
  • to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing

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