219466
CELLULYSIN™ Cellulase, Trichoderma viride
Synonym(s):
β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
biological source
fungus (Trichoderma viride)
form
solid
specific activity
≥10,000 units/g dry wt.
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
transformation: suitable
color
yellow-white
solubility
water: soluble
suitability
suitable for molecular biology
application(s)
food and beverages
pharmaceutical
shipped in
ambient
storage temp.
2-8°C
Application
CELLULYSIN™ Cellulase, Trichoderma viride has been used:
- to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
- in the enzyme mix used for in situ hybridization to plant chromosomes
- in the enzyme solution for the transformation of Phytophthora species
- to isolate intact cells and protoplasts from plant tissues
Biochem/physiol Actions
Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.
Disclaimer
Toxicity: Harmful (C)
General description
Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.
Other Notes
One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.
Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing
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