219466
CELLULYSIN™ Cellulase, Trichoderma viride
Synonym(s):
β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
eCl@ss:
32160410
Specific activity:
≥10,000 units/g dry wt.
Biological source:
fungus (Trichoderma viride)
biological source
fungus (Trichoderma viride)
form
solid
specific activity
≥10,000 units/g dry wt.
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
transformation: suitable
color
yellow-white
solubility
water: soluble
suitability
suitable for molecular biology
application(s)
food and beverages
pharmaceutical
shipped in
ambient
storage temp.
2-8°C
General description
Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.
Application
CELLULYSIN™ Cellulase, Trichoderma viride has been used:
- to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
- in the enzyme mix used for in situ hybridization to plant chromosomes
- in the enzyme solution for the transformation of Phytophthora species
- to isolate intact cells and protoplasts from plant tissues
Biochem/physiol Actions
Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.
Other Notes
One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.
Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 219466-200KU | 04055977218473 |
| 219466-100KU | 04055977218466 |
| 219466-50KU | 07790788048723 |