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219466

CELLULYSIN^™ Cellulase, Trichoderma viride

Name: CELLULYSIN<SUP>™</SUP> Cellulase, <i>Trichoderma viride</i>
Brand: MM

Synonym(s):

β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase

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About This Item

CAS Number:

9012-54-8

EC Number:

3.2.1.4 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD00081510

eCl@ss:

32160410

Specific activity:

≥10,000 units/g dry wt.

Biological source:

fungus (Trichoderma viride)

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Properties

biological source

fungus (Trichoderma viride)

form

solid

specific activity

≥10,000 units/g dry wt.

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

technique(s)

transformation: suitable

color

yellow-white

input

plant, fungi and insect sample(s)

solubility

water: soluble

suitability

suitable for molecular biology

application(s)

food and beverages
pharmaceutical

shipped in

ambient

storage temp.

2-8°C

Description

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Application

CELLULYSIN^™ Cellulase, Trichoderma viride has been used:

to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
in the enzyme mix used for in situ hybridization to plant chromosomes
in the enzyme solution for the transformation of Phytophthora species
to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P304 + P340 + P312 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)