234153
Collagenase, Type I, Clostridium histolyticum
Collagenase, Type I, Clostridium histolyticum, CAS 9001-12-1, is a crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities.
Synonym(s):
Collagenase, Type I, Clostridium histolyticum
form
lyophilized
specific activity
≥125 ABC units/mg solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
solubility
aqueous buffer: 1 mg/mL
foreign activity
Caseinase ≤100 FFC unit/500 ABC unit
shipped in
ambient
Quality Level
General description
Crude enzyme preparation that contains several collagenases, a sulfhydryl protease, clostripain and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies. Most active on fat cells, adrenal and liver tissue, with an optimal pH of 7.0-8.0.
Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies.
Note: 1 ABC unit = 1.1 BTC unit.
Other Notes
Harris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med.291, 557.
Seifter, S., and Harper, E. 1970. Methods Enzymol.19, 613.
Seifter, S., and Harper, E. 1970. Methods Enzymol.19, 613.
One ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
Storage Class
10-13 - German Storage Class 10 to 13
Regulatory Information
监管及禁止进口产品
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