251765
DAR-4M AM
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
Synonym(s):
DAR-4M AM, Diaminorhodamine-4M AM
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About This Item
Empirical Formula (Hill Notation):
C28H31IN4O5
Molecular Weight:
630.47
UNSPSC Code:
12352202
NACRES:
NA.25
Quality Level
Assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
dark red
fluorescence
λex ~560 nm
λem ~575 nm
shipped in
ambient
storage temp.
−20°C
Related Categories
General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
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