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Merck
CN

341631

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

Synonym(s):

Fibronectin, Bovine Plasma

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75
Form:
solution
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Product Name

Fibronectin, Bovine Plasma, solution, Extracellular matrix (ECM) glycoprotein, Suitable for cell adhesion, growth, migration, and differentiation.

Quality Level

description

Merck USA index - 14, 4075

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−20°C

General description

Fibronectin is an extracellular matrix (ECM) glycoprotein. It is expressed in various cell types. Fibronectin exists as a dimer and is made up of identical subunits which are linked covalently via a disulfide bond at their C-termini.

Application

Fibronectin, Bovine Plasma has been used as an extracellular matrix (ECM) substrate to coat dishes or polyacrylamide hydrogels for adhering:
  • human melanoma cancer cells
  • human breast epithelial cells
  • mouse embryonic fibroblasts

Biochem/physiol Actions

Fibronectin plays a role in cell adhesion, growth, migration, and differentiation. It binds to several molecules such as integrins, heparin, fibrin, and collagen.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

single band by SDS-PAGE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Purva Singh et al.
Annual review of cell and developmental biology, 26, 397-419 (2010-08-10)
In the process of matrix assembly, multivalent extracellular matrix (ECM) proteins are induced to self-associate and to interact with other ECM proteins to form fibrillar networks. Matrix assembly is usually initiated by ECM glycoproteins binding to cell surface receptors, such
Jordi Pijuan et al.
Frontiers in cell and developmental biology, 7, 107-107 (2019-07-02)
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells
Guillaume Jacquemet et al.
The Journal of cell biology, 216(10), 3387-3403 (2017-08-03)
Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack



Global Trade Item Number

SKUGTIN
341631-5MG04055977194906
341631-1MG04055977194883