341631
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
Synonym(s):
Fibronectin, Bovine Plasma
Product Name
Fibronectin, Bovine Plasma, solution, Extracellular matrix (ECM) glycoprotein, Suitable for cell adhesion, growth, migration, and differentiation.
Quality Level
description
Merck USA index - 14, 4075
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
dry ice
storage temp.
−20°C
General description
Fibronectin is an extracellular matrix (ECM) glycoprotein. It is expressed in various cell types. Fibronectin exists as a dimer and is made up of identical subunits which are linked covalently via a disulfide bond at their C-termini.
Application
Fibronectin, Bovine Plasma has been used as an extracellular matrix (ECM) substrate to coat dishes or polyacrylamide hydrogels for adhering:
- human melanoma cancer cells
- human breast epithelial cells
- mouse embryonic fibroblasts
Biochem/physiol Actions
Fibronectin plays a role in cell adhesion, growth, migration, and differentiation. It binds to several molecules such as integrins, heparin, fibrin, and collagen.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
single band by SDS-PAGE
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Purva Singh et al.
Annual review of cell and developmental biology, 26, 397-419 (2010-08-10)
In the process of matrix assembly, multivalent extracellular matrix (ECM) proteins are induced to self-associate and to interact with other ECM proteins to form fibrillar networks. Matrix assembly is usually initiated by ECM glycoproteins binding to cell surface receptors, such
Jordi Pijuan et al.
Frontiers in cell and developmental biology, 7, 107-107 (2019-07-02)
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells
Guillaume Jacquemet et al.
The Journal of cell biology, 216(10), 3387-3403 (2017-08-03)
Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack
Joseph A Brazzo et al.
Journal of cell science, 134(12) (2021-06-22)
Cell cycle control is a key aspect of numerous physiological and pathological processes. The contribution of biophysical cues, such as stiffness or elasticity of the underlying extracellular matrix (ECM), is critically important in regulating cell cycle progression and proliferation. Indeed
Patrizia M Spoerri et al.
Nature materials, 19(2), 218-226 (2020-01-22)
Haematopoietic cells and platelets employ G-protein-coupled receptors (GPCRs) to sense extracellular information and respond by initiating integrin-mediated adhesion. So far, such processes have not been demonstrated in non-haematopoietic cells. Here, we report that the activation of protease-activated receptors PAR1 and
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