341635
Human Fibronectin
from human plasma, liquid, suitable for cell culture
Synonym(s):
Fibronectin, Human Plasma, Fibronectin purified from human plasma, Human Fibronectin
Product Name
Fibronectin, Human Plasma, solution, suitable for cell culture
description
Merck USA index - 14, 4075
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Analysis Note
single major band by SDS-PAGE
Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum
Application
The human plasma fibronectin has been used for coating surfaces such as poly-l-lysine (PLL)-coated nanochips, polyacrylamide (PAAm) hydrogel substrates, glass slides and more, providing a substrate for the adhesion and culture of cells in various experimental setups.
Biochem/physiol Actions
Human fibronectin protein plays crucial roles in various cellular behaviors such as cell attachment, migration, embryogenesis, wound healing, tissue integrity, and malignant processes. One of the key features enabling fibronectin to support cell adhesion is the presence of the Arg-Gly-Asp (RGD) tripeptide. It has been extensively studied in areas like fibronectin coating on polystyrene (PS) and poly(lactic-co-glycolic acid) (PLGA) surfaces. Additionally, fibronectin has been the subject of significant research regarding its involvement in regulating processes such as differentiation-to-growth switching, tubulin monomer growth, cell cycle status, cell spreading, and motility.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
General description
Fibronectin is a glycoprotein belonging to the extracellular matrix (ECM) protein family. Fibronectin contains two polypeptides (α and β chains) linked by two disulfide bonds near the carboxyl termini to form a dimer.Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Other Notes
Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.
Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.
Brown, S., et al. 2002. Nature418, 200.
Kim, M., FEBS Letters581 1917.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.
Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.
Brown, S., et al. 2002. Nature418, 200.
Kim, M., FEBS Letters581 1917.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.3.
Preparation Note
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments
Pietrocola G, et al.
Journal of Separation Science, 1627, 309-324 (2017)
Design of artificial extracellular matrices for tissue engineering
Kim, Byung-Soo and Park, et al.
Progress in Polymer Science, 36(2) (2011)
Jian Wu et al.
Cells, 12(15) (2023-08-11)
Retinoid X receptor (RXR) heterodimerizes with the PPAR nuclear hormone receptor and regulates its downstream events. We investigated the effects of RXR agonists (LG100754, bexarotene, AGN194204, and LG101506) on lenalidomide's anti-myeloma activity, T cell functions, and the level of glucose
Monica L Salgado-Lucio et al.
Journal of cell science, 133(8) (2020-02-29)
Actin polymerization is a crucial process during sperm capacitation. We have recently described the participation of FAK during actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK mediates these processes is unknown. Our previous data have shown
Wei Zhang et al.
Nature cell biology, 25(10), 1453-1464 (2023-09-29)
Integrin-mediated focal adhesions are the primary architectures that transmit forces between the extracellular matrix (ECM) and the actin cytoskeleton. Although focal adhesions are abundant on rigid and flat substrates that support high mechanical tensions, they are sparse in soft three-dimensional
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