345795
β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli
Synonym(s):
β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli, β-D-Galactoside Galactohydrolase
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recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥2000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
β-N-acetylglucosaminidase, α-fucosidase, α-galactosidase, β-glucosidase, α- and β-mannosidase, proteases, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3- over β1,6-linkages and 500-fold kinetic preference for β1,3- over β1,4-linkages.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 100 µM EDTA, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will cleave >95% of the β-D-galactose from 1 nmol Galβ1,3GlcNAcβ1,3Galβ1,4Glc-7-amino-4-methylcoumarin in 1 h at 37°C, pH 4.5 in a total volume of 10 µl.
Taron, C., et al. 1995. Glycobiology 5, 603.
Wong-Madden, S.T., and Landry, D. 1995. Glycobiology 5, 19.
Wong-Madden, S.T., and Landry, D. 1995. Glycobiology 5, 19.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 345795-500U | 04055977194029 |