361524
GSK-3β, His•Tag®, Human, Recombinant, E. coli
GSK-3β, His-Tag, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
Synonym(s):
GSK-3β, Tau Protein Kinase I, TPK I
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About This Item
UNSPSC Code:
12352202
Form:
solid
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human GSK-3β fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer′s disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB. MW: 53,000-56,000
Biochem/physiol Actions
1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
Physical form
Lyophilized from 0.1 M NH₄CO₃.
Preparation Note
Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute in sterile PBS to a final concentration of 1 mg/ml.
Other Notes
Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol.5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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Brian L Olson et al.
Genes & development, 22(2), 252-264 (2008-01-17)
Peroxisome proliferator-activated receptor gamma (PPARgamma) coactivator-1alpha (PGC-1alpha) is a highly regulated transcriptional coactivator that coordinates energy metabolism in mammals. Misregulation of PGC-1alpha has been implicated in the pathogenesis of several human diseases, including diabetes, obesity, and neurological disorders. We identified
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