382160
Anti-Histone H4, Acetylated (2-19) Rabbit pAb
liquid, Calbiochem®
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
Tetrahymena sp., human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Anti-Histone H4, Acetylated (2-19), rabbit polyclonal, recognizes the ~10 kDa acetylated histone H4 in Tetrahymena and sodium butyrate-treated HeLa cells. It is validated for use in WB, ICC, and IP.
Protein A purified rabbit polyclonal antibody. Recognizes the ~10 kDa acetylated histone H4 protein.
Recognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.
Immunogen
Tetrahymena
a tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
Application
Immunoblotting (0.5-2 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (5µg/500 µg lysate)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (5µg/500 µg lysate)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Tetrahymena and sodium butyrate-treated HeLa cells
Tetrahymena and sodium butyrate-treated HeLa cells
Other Notes
Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
The antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10-13 - German Storage Class 10 to 13
Regulatory Information
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