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Merck
CN

386041

Sigma-Aldrich

Anti-Hsp90 Rat mAb (16F1)

liquid, clone 16F1, Calbiochem®

Synonym(s):

Anti-Hsp82, Anti-Hsp85, Anti-Hsp89

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

16F1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSP90AA1(3320)

General description

Protein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Rat mAb (16F1) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Electron Microscopy for the detection of Hsp90.

Immunogen

Hsp90 isolated from therapeutic human orchiectomy specimens
Human

Application

ELISA (see comments)

Immunoblotting (2 µg/ml, colormetric)

Immunocytochemistry (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Electron Microscopy (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Heat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells

Other Notes

Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
Lanks, K.W., et al. 1982. J. Biol. Chem.257, 8604.
This antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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