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400064

Anti-Glucose Transporter-4 Rabbit pAb

Name: Anti-Glucose Transporter-4 Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Glut-4

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

rat, human, mouse

Application:

—

Citations:

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Properties

biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (0.5-1 µg/mL)
Frozen Sections (2-20 µg/mL)
Immunoblotting (1-10 µg/mL)
Immunoprecipitation (1-10 µg/100 µg tissue membrane))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC2A4(6517)

Description

General description

Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.

Recognizes the ~40-43 kDa GLUT-4 protein.

Immunogen

Mouse

a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH

Application

ELISA (0.5-1 µg/ml)

Frozen Sections (2-20 µg/ml)

Immunoblotting (1-10 µg/ml; see comments)

Immunoprecipitation (1-10 µg/100 µg tissue membrane)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.1% BSA, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.

Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c