407251
Insulin-Like Growth Factor-I, Human, Recombinant, E. coli, Animal-Free
Synonym(s):
hIGF-I
Assay
≥98% (SDS-PAGE and HPLC)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.1 ng/μg Endotoxins
shipped in
wet ice
storage temp.
−20°C
General description
Full-length, recombinant, human insulin-like growth factor I (IGFI) expressed in E. coli. The IGFI growth factor regulates proliferation and survival signals through the AKT pathway. This animal free growth hormone is useful in the study of cell proliferation and survival signals.
Biochem/physiol Actions
ED ₅₀: ≤1 ng/ml as determined by a cell proliferation assay using FDC-P1 cells; this corresponds to a specific ≥1 x 10⁶ units/mg.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute with H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with physiological buffer prior to use.
Other Notes
Stabnov, L., et al. 2002. Bone30, 909.
Devireddy, L.R., et al. 2001. Science 293, 829.
Chen, C.W., and Ray, D. 1995. Carcinogenesis 16, 1339.
Devireddy, L.R., et al. 2001. Science 293, 829.
Chen, C.W., and Ray, D. 1995. Carcinogenesis 16, 1339.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Longxing Cao et al.
Nature, 605(7910), 551-560 (2022-03-26)
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1-5. Here we describe a general solution to this problem
Blaz Lupse et al.
Cell reports, 36(5), 109490-109490 (2021-08-05)
Pancreatic β-cell failure is the key pathogenic element of the complex metabolic deterioration in type 2 diabetes (T2D); its underlying pathomechanism is still elusive. Here, we identify pleckstrin homology domain leucine-rich repeat protein phosphatases 1 and 2 (PHLPP1/2) as phosphatases
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