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Merck
CN

407623-M

Sigma-Aldrich

Interleukin-2, Human, Recombinant, E. coli

Synonym(s):

rhIL-2

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About This Item

UNSPSC Code:
12352202

Key Documents

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Assay

≥95% (SDS-PAGE)

Quality Level

100

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤100 pg/μg Endotoxin (pg/μg IL-2)

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human interleukin-2 expressed in E. coli. Has a variety of immunological functions. IL-2 is secreted by activated T cells. Acts as an autocrine agent to promote proliferation and maturation of activated T cells. Human IL-2 is active in murine cell lines. Exhibits about 60% amino acid homology with murine IL-2. Inhibits dexamethasone-induced apoptosis in T lymphocytes. Induces nitric oxide synthase.

Recombinant, human interleukin-2 expressed in E. coli. Has a variety of immunological functions. Acts as an autocrine agent to promote proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows ~60% sequence similarity. Inhibits dexamethasone-induced apoptosis in T-lymphocytes. Induces nitric oxide synthase. Human IL-2 is active in murine cell lines. Validated for use in cell culture and bioassays.

Biochem/physiol Actions

ED₅₀= 80-500 pg/ml as measured in a cell proliferation assay with an IL-2-dependent murine cytotoxic T cell line

Physical form

Carrier-free

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration of 0.1-1 mg/ml with sterile 100 mM acetic acid. Further dilute in buffer or medium containing ≥0.1% BSA.

Other Notes

Migliorati, G., et al. 1994. Pharmacol. Res.30, 43.
Nakamura, Y., et al. 1994. Cancer Res. 54, 5757.
Karanth, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3383.
Maeda, A., et al. 1983. Biochem. Biophys. Res. Commun.115, 1040.
Taniguchi, T., et al. 1983. Nature302, 305.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Regulatory Information

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