GST fusion protein of c-jun (1-79) activation domain, expressed in E. coli. Highly specific substrate form c-jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Not phosphorylated by MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity.

Recombinant c-Jun (1-79) activation domain fused to GST and expressed in E. coli. Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Is not phosphorylated by p42 MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity. Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.

Cell permeable: no

Primary Target
Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)

Product does not compete with ATP.

Reversible: no

Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.

In 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Derijard, B., et al. 1994. Cell 76, 1025.
Kyriakis, J.M., et al. 1994. Nature369, 156.
Hibi, M., et al. 1993. Genes Dev.7, 2135.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)