487600-M
p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem
Each 175 mg tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt plus an inert filler.
Synonym(s):
p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem, pNPP, 2Na
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About This Item
Empirical Formula (Hill Notation):
C6H4NO6P · 2Na · 6H2O
CAS Number:
Molecular Weight:
371.14
UNSPSC Code:
12352200
MDL number:
Quality Level
form
tablet
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
color
light yellow
storage temp.
2-8°C
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
General description
Each 175 mg tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt (Cat. No. 4876) plus an inert filler. Dissolve one tablet in 5 ml of 10 mM diethanolamine, pH 9.5 containing 500 µM MgCl2, to prepare a 1 mg/ml solution.
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. Each tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt (cat. no. 4876) plus an inert filler.
Note: 1 each = 100 tablets.
Biochem/physiol Actions
Primary Target
Substrate for phosphatase
Substrate for phosphatase
Preparation Note
Dissolve one tablet in 5 ml of of 10 mM diethanolamine, pH 9.5 containing 0.5 mM MgCl₂ to prepare a 1 mg/ml stock solution.
Unstable in solution; reconstitute just prior to use. Following reconstitution, aliquot and refrigerate (4°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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